High Tech with a Human Touch
CARROLL, David J
Associate Professor and Director of Graduate Programs in Biological Sciences
Biological Sciences Department, College of Science
B.S., Microbiology Auburn University 1988
M.S., Zoology Auburn University 1991
Ph.D., Biomedical Science University of Connecticut 1996
Director of Graduate Programs in Biological Sciences, Florida Institute of Technology, Melbourne, FL. 2010 - present
Associate Professor, Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL.
2005 - present
Assistant Professor, Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL.
1999 - 2005
NIH NRSA Postdoctoral Research Fellow with Dr. Kathy Foltz, Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA. 1997 - 1999
Associate Editor BMC Developmental Biology
2008 - present
Using a combination of biochemistry, molecular biology and live cell microscopy, we have discovered that PLCg is required for the initiation of the calcium increase during fertilization in starfish and sea urchin eggs. In collaboration with Dr. Kathy Foltz of the University of CaliforniaÂ–Santa Barbara and Dr. Laurinda Jaffe of the University of Connecticut Health Center, we have recently cloned a starfish PLCg cDNA from an arrayed oocyte cDNA library. We are currently using this clone as a tool to look for proteins that interact with PLC? in a fertilization-specific manner. We have determined that there are a suite of tyrosine-phosphorylated proteins, some from the sperm and some from the egg, that bind to PLCg only after fertilization. The identification of these proteins is a major goal of the laboratory in the coming years.
McGinnis, LK, Carroll, DJ, Kinsey, WH (2011) Protein tyrosine kinase signaling during oocyte maturation and fertilization. Mol. Reprod. Dev. 78:831-45.
Stricker, S.A., Carroll, D.J., Tsui, W.L. (2010) Roles of Src Family Kinase signaling and the first cell cycle in the marine protostome worm Cerebratulus. Int. J. Dev. Biol. 54:787-93.
Carroll, D.J. and Wei, H. (2009) Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs. Methods Mol. Biol. 518, 57 -66.
*Runft, L.R., *D.J. Carroll, *J. Gillett, A.F. Giusti, F.J. ONeill and K.R. Foltz. 2004. Identification of a Starfish Egg PLC gamma that regulates Ca2+ release at fertilization. Dev. Biol. 269 (May 1): 220-236. *These 3 authors contributed equally to the project and manuscript.
Kumano, M., D.J. Carroll, J.R. Denu and K.R. Foltz. 2001. Calcium-mediated inactivation of the MAP kinase pathway in sea urchin eggs at fertilization.
Dev. Biol. 236:244-257.
Carroll, D.J., D.T. Albay, K. Hoang, F.J. ONeill, M. Kumano and K.R. Foltz. 2000. The relationship between calcium, MAP kinase, and the initiation of DNA synthesis in the sea urchin egg at fertilization. Dev. Biol. 217:179-191.