Christopher Chouinard

Assistant Professor | Biomedical and Chemical Engineering and Sciences

Contact Information

Personal Overview

Dr. Chouinard was born and raised in the small town of Grafton, MA. His interests in chemistry and biology brought him south, where he attended University of North Carolina at Chapel Hill (Go Tar Heels!) and furthered his passion for analytical chemistry. After briefly working as a project manager for a contract research lab back in Massachusetts, Chris pursued his Ph.D. at the University of Florida. There, Chris worked under world renowned mass spectrometrist Rick Yost, and was responsible for integrating drift tube-ion mobility spectrometry (DTIMS) into the Yost lab. Chris's interests at UF involved IM-MS studies of clinical molecules (steroids, Vitamin D metabolites) and he also received grant funding through the Partnership for Clean Competition to develop novel IM-MS methods for improved identification of anabolic androgenic steroids in athletes.

After graduating from UF, Chris traveled to the opposite corner of the country to engage in a prestigious post-doctoral position at Pacific Northwest National Laboratory (PNNL) in Richland, WA. While working with Dick Smith, another world leader in mass spectrometry, Chris designed and developed a Structures for Lossless Ion Manipulations (SLIM) platform that enabled the first integration of LC into the SLIM IM-MS pipeline for improved analysis in phosphoproteomics.

Chris joined the faculty at Florida Tech in the summer of 2018 and will continue his work in ion mobility-mass spectrometry. Current projects involve improved methods for metabolomics and lipidomics with gas-phase chemistry.

Educational Background

University of Florida; Gainesville, FL August 2012 – August 2016
Ph.D., Analytical Chemistry
Research Advisor – Dr. Richard A. Yost

University of North Carolina at Chapel Hill; Chapel Hill, NC August 2006 – May 2010
B.S. Chemistry; B.S. Biology
Research Advisor – Dr. Mark H. Schoenfisch

Professional Experience

Florida Institute of Technology; Melbourne, FL August 2018 - Present
Assistant Professor, Chemistry

Pacific Northwest National Laboratory (PNNL); Richland, WA September 2016 - June 2018
Post-Doctoral Research Associate
Research Advisor – Dr. Richard D. Smith

Current Courses

CHM 3301: Analytical Chemistry 1 - Quantitative Analysis 
CHM 3302: Analytical Chemistry 2 - Instrumental Analysis 
CHM 5305: Special Topics in Analytical Chemistry - Mass Spectrometry
CHM 4304/5304: Advanced Analytical Chemistry

Selected Publications

  1. Katie M. Wilson; Aurora Burkus-Matesevac; Samuel W. Maddox; Christopher D. Chouinard.  “Native Ubiquitin Structural Changes Resulting from Complexation with β-methylamino-L-alanine (BMAA),” J Amer Soc Mass Spectrom, 202132, 895-900. DOI: 10.1021/jasms.0c00372
  2. Samuel M. Maddox; Stine S.H. Olsen; Diana C. Velosa; Aurora Burkus-Matesevac; Roberto Peverati; Christopher D. Chouinard. “Improved Identification of Isomeric Steroids using the Paternò-Büchi Reaction with Ion Mobility-Mass Spectrometry,” Journal of the American Society for Mass Spectrometry, 202031, 2086-2092. DOI: 10.1021/jasms.0c00215
  3. Samuel M. Maddox; Robert H. Fraser Caris; Kristie L. Baker; Aurora Burkus-Matesevac; Roberto Peverati; Christopher D. Chouinard. “Ozone-Induced Cleavage of Endocyclic C=C Double Bonds within Steroid Epimers Produces Unique Gas-Phase Conformations,” Journal of the American Society for Mass Spectrometry, 202031, 411-417. DOI: 10.1021/jasms.9b00058
  4. Pierpaolo Morgante; Burjor Captain; Christopher D. Chouinard; Roberto Peverati; Norito Takenaka. “Synthesis of electrophilic N-heterocyclic carbenes based on azahelicene,” Tetrahedron Letters202061, 152143. DOI: 10.1016/j.tetlet.2020.152143
  5. Ailin Li; Gabe Nagy; Christopher Conant; Randolph Norheim; Joon-Yong Lee; Cameron Giberson; Adam Hollerbach; Venkateshkumar Prabhakaran; Isaac Attah; Christopher D. Chouinard; Aneesh Prabhakaran; Richard Smith; Yehia Ibrahim; Sandilya V.B. Garimella. “Ion Mobility Spectrometry With High Ion Utilization Efficiency Using Traveling Wave-Based Structures For Lossless Ion Manipulations (SLIM)", Analytical Chemistry2020In Press, DOI: 10.1021/acs.analchem.0c02100
  6. Eric W. Ziegler; Alan B. Brown; Nasri Nesnas; Christopher D. Chouinard; Anil K. Mehta; Andrew G. Palmer. “β-Cyclodextrin encapsulation of synthetic AHLs: drug delivery implications and quorum-quenching exploits,” Chem Bio Chem, 2020In Press. DOI: 10.1002/cbic.202000773
  7. Nicholas R. Oranzi; Jiajun Lei; Robin H.J. Kemperman; Christopher D. Chouinard; Brett Holmquist; Timothy J. Garrett; Richard A. Yost. “Rapid Quantitation of 25-Hydroxyvitamin D2 and D3 in Human Serum Using Liquid Chromatography/drift Tube Ion Mobility-Mass Spectrometry,” Analytical Chemistry2019, 90, 13549-13556. DOI: 10.1021/acs.analchem.8b03668
  8. Christopher D. Chouinard; Maowei Dou; Ying Zhu; Gabe Nagy; Andrey V. Liyu; Yehia M. Ibrahim; Richard D. Smith; Ryan T. Kelly. “Nanowell-Mediated Multidimensional Separations Combining nanoLC with SLIM IM-MS for Rapid, High-Peak-Capacity Proteomic Analyses,” 2019Analytical and Bioanalytical Chemistry, 411, 5363-6372. DOI: 10.1007/s00216-018-1452-5
  9. Christopher D. Chouinard; Gabe Nagy; Ian K. Webb; Tujin Shi; Erin S. Baker; Spencer A. Prost; Tao Liu; Yehia M. Ibrahim; Richard D. Smith. “Improved Sensitivity and Separations of Phosphopeptides using Online LC Coupled with Structures for Lossless Ion Manipulations (SLIM) IM-MS,” Analytical Chemistry, 2018, 90, 10889-10896DOI: 10.1021/acs.analchem.8b02397
  10. Christopher D. Chouinard; Gabe Nagy; Ian K. Webb; Sandilya V.B Garimella; Erin S. Baker; Yehia M. Ibrahim; Richard D. Smith. “Rapid Ion Mobility Separations of Bile Acid Isomers using Cyclodextrin Adducts and Structures for Lossless Ion Manipulations,” Analytical Chemistry2018, 90, 11086-11091. DOI: 1021/acs.analchem.8b02990
  11. Gabe Nagy; Christopher D. Chouinard; Isaac K. Attah; Ian K. Webb; Sandilya V.B. Garimella; Yehia M. Ibrahim; Richard D. Smith; Erin S. Baker. “Distinguishing Enantiomeric Amino Acids with Chiral Cyclodextrin Adducts and Structures for Lossless Ion Manipulations,” Electrophoresis, 2018, 39 (24), 3148-3155. DOI: 10.1002/elps.201800294
  12. Rhea C. Garcellano, Syed G.A. Moinuddin, Mark E. Bowden, Ryan S. Renslow, Yasemin Yesiltepe, Dennis G. Thomas, Robert P. Young, Christopher D. Chouinard, Gabe Nagy, Isaac K. Attah, Yehia Ibrahim, Mowei Zhou, Rui Ma, Scott G. Franzblau, Norman G. Lewis, Alicia M. Aguinaldo, John R. Cort. “Isolation of Tryptanthrin and Reassessment of Evidence for its Isobaric Isostere Wrightiadione in Plants of the Wrightia Genus,” 2018Journal of Natural ProductsIn Press. DOI: 10.1021/acs.jnatprod.8b00567
  13. Christopher D. Chouinard; Vinicius W.D. Cruzeiro; Robin H.J. Kemperman; Nicholas R. Oranzi; Adrian E. Roitberg; Richard A Yost. “Cation-Dependent Conformations in 25-Hydroxyvitamin D3-Cation Adducts Measured by Ion Mobility-Mass Spectrometry and Theoretical Modeling,” International Journal of Mass Spectrometry2018, 432, 1-8. DOI: 10.1016/j.ijms.2018.05.013
  14. Christopher D. Chouinard; Vinicius W.D. Cruzeiro; Adrian E. Roitberg; Richard A Yost. “Experimental and Theoretical Investigation of Sodiated Multimers of Steroid Epimers with Ion Mobility-Mass Spectrometry,” Journal of the American Society for Mass Spectrometry2017, 28, 323-331. DOI: 10.1007/s13361-016-1525-7
  15. Christopher D. Chouinard; Christopher R. Beekman; Robin H.J. Kemperman; Harrison M. King; Richard A Yost. “Ion Mobility-Mass Spectrometry Separation of Steroid Structural Isomers and Epimers,” International Journal for Ion Mobility Spectrometry, 2017, 20, 31-39. DOI: 10.1007/s12127-016-0213-4
  16. Christopher D. Chouinard; Vinicius W.D. Cruzeiro; Christopher R. Beekman; Adrian E. Roitberg; Richard A Yost. “Investigating Differences in Gas-Phase Conformations of 25-Hydroxyvitamin D3 Sodiated Epimers using Ion Mobility-Mass Spectrometry and Theoretical Modeling,” Journal of the American Society for Mass Spectrometry, 2017, 28, 1497-1505. DOI: 10.1007/s13361-017-1673-4
  17. Christopher D. Chouinard; Michael S. Wei; Christopher R. Beekman; Robin H.J. Kemperman; Richard A Yost. “Ion Mobility in Clinical Analysis: Current Progress and Future Perspectives,” Clinical Chemistry2016, 62, 124-133. Invited Special Issue; DOI: 10.1373/clinchem.2015.238840

Recognition & Awards

2012-16 University Graduate Student Fellowship Recipient
2013      Townes R. Leigh Award for Outstanding Achievement by First Year Graduate Student
2015      Eastman Chemical Company Summer Fellowship
2016      Laitinen Award for Overall Excellence in Analytical Chemistry, UF
2016-17 MSACL US Young Investigator Award
2018      Agilent Steve Berger Award
2018      Agilent Global Academic Research Support Program

Research

Our group uses ion mobility-mass spectrometry (IM-MS) for characterization of small molecules in bioanalytical applications. Funded projects include development of novel methods for detection of performance-enhancing drugs (PEDs), identification of catalytic breakdown products of opioids, and measurement of environmental contaminants and their effects on human health. Check out our research website for more information!