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Robert J. Usselman

Assistant Professor | Biomedical and Chemical Engineering and Sciences

h-index (16) | biophysical chemistry, cell biology, redox biochemistry, proteins, magnetic resonance, quantum biology and sensing, deep learning and A.I. in biochemistry, microscopy

Contact Information

Educational Background

2005 Ph.D. Bio-Physical Chemistry, Montana State University, Bozeman, MT  

1999 B.A. in Chemistry (ACS-certified), Mathematics Minor Concordia College – Moorhead, MN

Professional Experience

2018-present Assistant Professor

Department of Chemistry and Chemical Engineering, Florida Institute of Technology, Melbourne, FL

2014-2018 Assistant Research Professor

Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT

2010-2014 Senior Research Scientist

Magnetics Group, Electromagnetics Division, Physical Measurement Laboratory, NIST

2010-2014 Research Faculty

Professional Research Experience Program (PREP), University of Colorado – Boulder, CO

2008-2010

National Research Council (NRC) Postdoctoral Fellowship

Magnetics Group, Electromagnetics Division, Physical Measurement Laboratory, NIST

2006-2008 Postdoctoral Associate

University of Denver, University of Colorado Health Science Center, Denver, CO

Current Courses

CHM 1101

CHM 1102

Analytical CHM Lab 3311

Selected Publications

22). Quantum Biology in Cellular Migration, Amy Vecheck, Cameron McNamee, Renee Reijo-Pera, Robert J. Usselman, BioRχiv (2022)

21). Blue-light Induced Accumulation of Reactive Oxygen Species is a Consequence of the Drosophila Cryptochrome Photocycle, Louis-David Arthaut, Nathalie Jourdan, Ali Mteyrek, Maria Procopio, Mohamed El-Esawi, Alain d’Harlingue, Pierre-Etienne Bouchet, Jacques Witczak, Thorsten Ritz, Andre Klarsfeld, Serge Birman, Ute Hoecker, Robert J. Usselman, Carlos F. Martino, and Margaret Ahmad, PLoS ONE 12(3): e0171836 (2017).

 20). Electron Spin Relaxation and Biochemical Characterization of the Hydrogenase Maturase HydF: Insights into [2Fe-2S] and [4Fe-4S] Cluster Communication and Hydrogenase Activation, Eric Shepard, Priyanka Aggarwal, Amanda Byer, Jerimiah Betz, Anna Scott, Krista Shisler, Sandra Eaton, Gareth Eaton, Robert J. Usselman, and Joan Broaderick, Biochemistry 56 (25), 3234-3247 (2017).

19). The Quantum Biology of Reactive Oxygen Species Partitioning Impacts Cellular Bioenergetics, Robert J. Usselman, Christina Chavarriaga, Pablo Castello, Maria Procopio, Thorsten Ritz, Edward Dratz, David J. Singel, and Carlos Martino, Scientific Reports 6: 38543 (2016).

 18). Monooxygenase Substrates Mimic Flavin to Catalyze Cofactorless Oxygenations, Melody M. Machovina, Robert J. Usselman, Jennifer L. DuBois, Journal of Biological Chemistry, jbc.M116.730051 (2016).

 17). On-wafer Magnetic Resonance of Magnetite Nanoparticles, Charles E. Little, Stephen E. Russek, James C. Booth, Pavol Kabos, and Robert J. Usselman, Journal of Magnetism and Magnetic Materials 393, 15-19 (2015).

 16). Temperature-dependent Structure of Tb-doped Magnetite Nanoparticles, Katherine Rice, Stephen E. Russek, Roy Geiss, Justin M. Shaw, Robert J. Usselman, Eric R. Evart, Tom J. Silva, Hans T. Nembach, and Yves Idzerda, Applied Physics Letters 106 (6), 062409 (2015).

15). Gadolinium-Loaded Viral Capsids as Magnetic Resonance Imaging Contrast Agents, Robert J. Usselman, Shefah Qazi, Priyanka Aggarwal, Sandra Eaton, Gareth Eaton, Trevor Douglas, and Stephen Russek, Applied Magnetic Resonance 46 (3), 349-355 (2015).

14). Dynamics of Magnetic Nanoparticles and Nanodevices, Stephen Russek, Robert J. Usselman, and Eric R. Evarts, Handbood of Nanomagnetism: Applications and Tools, CRC Press, Chapter 6, 117-173 (2015).

13). Inhibition of Cellular Proliferation and Enhancement of Hydrogen Peroxide Production in Fibrosarcoma Cell Line by Weak Radio Frequency Magnetic Fields, Pablo Castello, Lucas Portilli, Frank Barnes, Robert J. Usselman, and Carlos Martino, Bioelectromagnetics 35 (8), 598-602 (2014).

12). Spin Biochemistry Modulates Reactive Oxygen Species (ROS) Production by Radio Frequency Magnetic Fields, Robert J. Usselman, Iain Hill, David Singel, and Carlos Martino, PLoS ONE 9(3), e93065 (2014).

11). Manganese (III) Porphyrins Complexed with P22 Virus-like Particles as T1-enhanced Contrast Agents for Magnetic Resonance Imaging (MRI), Shefah, Qazi, Masaki Uchida, Robert J. Usselman, and Trevor Douglas, Journal of Inorganic Biochemistry February 19 (2), 237-246 (2014). 

10).  Temperature Dependence of Electron Magnetic Resonance Spectra of Iron Oxide Nanoparticles Mineralized in Listeria Innocua Protein Cages, Robert J. Usselman, Stephen E. Russek, Michael Klem, Mark Allen, Trevor Douglas, Mark Young, Yves Idzerda, and David Singel, Journal of Applied Physics 112, 084701 (2012).

9).  Citrate Mediated Wet Chemical Synthesis of Fe Doped Nanoapatites: A Model for Singly Doped Multi-function Nanostructures, Rajendra K. Kasinath, Michael T. Klem, and Robert J. Usselman, (2012) in Supplemental Proceedings: Materials Properties, Characterization, and Modeling, Volume 2 (ed TMS) John Wiley and Sons, Inc., Hoboken, NJ, USA.

8).  Monitoring Structural Transitions in Icosahedral Virus Protein Cages by Site-Directed Spin Labeling, Robert J. Usselman, Eric D. Walter, Debbie Willits, Trevor Douglas, Mark Young, and David J. Singel, Journal of American Chemical Society, 133 (12), 4156-4159 (2011).

7).  End-Group Distributions of Multiple Generations of Spin-Labeled PAMAM Dendrimers, Karl B. Sebby, Eric D. Walter, Robert J. Usselman, Mary J. Cloninger, and David J. Singel, Journal of Physical Chemistry B, 115 (16), 4613-4620 (2011).

6).  Two-Component Magnetic Structure of Iron Oxide Nanoparticles Mineralized in Listeria Innocua Protein Cages, Robert J. Usselman, Michael Klem, Trevor Douglas, Mark Young, Steve Russek, and Ron Goldfarb, Journal of Applied Physics 107 (11), 114702 (2010).

5).  The Iron-Sulfur Cluster of Electron Transfer Flavoprotein-Ubiquinone Oxidoreductase is the Electron Acceptor for Electron Transfer Flavoprotein, Michael Swanson, Robert J. Usselman, Frank E. Frerman, Gareth R. Eaton, and Sandra S. Eaton, Biochemistry. 47 (34), 8894-901 (2008).

4).  Impact of Mutations on Redox Potentials, g-Values, and Spin-Lattice Relaxation Rates of the [4Fe-4S]2+,1+ Cluster in ETF-QO, Robert J. Usselman, Alistair Fielding, Frank E. Frerman, Nick Watmough, Gareth R. Eaton, and Sandra S. Eaton, Biochemistry. 47 (1), 92-100 (2008).

3).  Electron Paramagnetic Resonance Characterization and Interspin Distance Measurement of Electron Transfer Flavoprotein‑ubiquinone Oxidoreductase (ETF-QO), Alistair J. Fielding, Robert J. Usselman, Frank E. Frerman, Gareth R. Eaton and Sandra S. Eaton, Journal of Magnetic Resonance 190 (2), 222-32 (2008).

2).  Characterization of Heterogeneously Functionalized Dendrimers by Mass Spectrometry and EPR Spectroscopy, Eric D. Walter, Karl B. Sebby, Robert J. Usselman, David J. Singel, and Mary J. Cloninger, J. Phys. Chem. B; 109 (46), 21532 – 21538 (2005).

1). Electron Magnetic Resonance of Iron Oxide Nanoparticles Mineralized in Protein Cages, Robert J. Usselman, Michael T. Klem, Mark Allen, Eric D. Walter, Keith Gilmore, Trevor Douglas, Mark Young, Yves Idzerda, David J. Singel, Journal of Applied Physics; 97, 10M523 (2005).

 

Recognition & Awards

2015 - American Heart Association Scientific Development Grant

2008 - National Research Council (NRC) Postdoctoral Fellowship NIST-Boulder

2001 - National Science Foundation Integrative Graduate Education and Research Traineeship (NSF-IGERT) Montana State University 

2004 - Student Travel Award, 27th International EPR Symposium, Denver

1999 - Graduated with Chemistry Honors, Concordia College

1998 - Ough Family Scholarship in Mathematics, Concordia College

Research

The Usselman research lab is always welcoming eager, inquisitive, and motivated undergraduate, graduate, and postdoctoral students to join our multidisciplinary research team at front-edge science.  Please email the Prof. Usselman for inquiries about the many research opportunities.  

The overarching theme of the Usselman research program is to use advanced magnetic resonance and optical microscopy techniques to address bio-physical chemistry problems in the areas of chemical biology, biomaterials, and redox biochemistry.  We aim to develop novel methodologies and instrumentation that address the fundamental gap in knowledge between physical measurements and theoretical models for bio-engineered systems operating at the quantum/classical interface.  We seek to understand how quantum properties play governing roles in biological function and apply theory-driven predictions of quantum biology for multi-scale integration of cellular function.

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